——Protein A/G Agarose or Magnetic Beads
Protein A and Protein G are the most commonly used proteins for antibody affinity purification and immunoprecipitation. Protein A/G agarose or magnetic beads covalently couple Protein A and G to the medium surface, featuring stronger binding capacity, a broader binding range, and higher practicality.
|
rProtein A/G Agarose Resin |
||
|
Matrix |
Highly cross-linked 4% agarose microspheres |
Polymeric magnetic microspheres |
|
Particle Size |
45-165 µm |
1 µm |
|
Capacity |
>20 mg hIgG/mL resin |
>0.7 mg hIgG/mg magnetic beads |
|
Advantages |
Easy to use, no auxiliary equipment required |
Simple operation |
|
Large pore size, strong binding force |
Small diameter, good kinetics |
|
|
High yield of target protein |
Smooth surface, minimal bead adsorption, low background, less antibody consumption |
|
|
Disadvantages |
Porous and prone to adsorption (pre-clear required) |
Convenient to use with a magnetic rack |
Product Applications
1.Purification of Antibodies
A single step of purification can isolate high-purity antibodies from serum or ascites samples.
Enrichment of a specific protein to study its characteristics (such as post-translational modifications).
3.Co-Immunoprecipitation (Co-IP)
Enrichment of a specific protein to study other proteins that bind to it (i.e., protein-protein interactions, protein complexes, etc.).
Operation Procedure
Test Data
1.Purification of antibodies from rabbit serum using rProtein A/GAgarose Resin.
Figure 1. Electrophoresis result of antibody purification using rProtein A/G Agarose Resin
2.Use of Protein A/G Magbeads for Co-IP: Specific antibodies against p65 and cyclin-dependent kinase 9 (CDK9) are used for immunoprecipitation analysis of extracted total cellular protein.
Figure 2. Immunoprecipitants were then subjected to western blot analysis using specific antibodies against CDK9 and p65.
Product Information
|
Product Name |
Product Number |
Specification |
|
rProtein A/G Agarose Resin |
36403ES08/25/60 |
5 mL/25 mL/100 mL |
|
36417ES03/05 |
1 mL/5 mL |
Related Products Order
|
Product Name |
Product Number |
Specification |
|
36401ES08/25/60 |
5 mL/25 mL/100 mL |
|
|
rProtein G Agarose Resin |
36405ES08/25/60 |
5 mL/25 mL/100 mL |