विवरण
Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo . Mature human ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with ANGPTL1, 2, 3, 5, 6, and 7. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers . This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels. In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL. In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL. Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity. Its expression in adipose tissue is induced by fasting and suppressed by feeding. In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells. The N-terminal fragment can function as an angiogenesis inhibitor. In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing. ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells . The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome.
Specifications
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Synonyms |
ANGPTL4; ARP4; FIAF; HFARP; Angiopoietin like 4; NGPTL2; NL2; PGAR; pp1158; ANG-3; ANG4; AGP4; ANG3; ANG-3; ANG-4; |
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Uniprot No. |
Q9BY76.1 |
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Source |
Recombinant Human ANGPTL4/Angiopoietin-like 4 Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Pro166-Ser406. |
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Molecular Weight |
Approximately 28.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Activity |
SPR Data: Human LILRB2 captured on Protein A chip, can bind Human ANGPTL4, His Tag with an affinity constant of 13.8nM as determined in a SPR assay (Biacore T200). |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
Product Data
भुगतान और सुरक्षा
आपकी भुगतान जानकारी सुरक्षित रूप से संसाधित की जाती है। हम क्रेडिट कार्ड के विवरण को संग्रहीत नहीं करते हैं और न ही आपके क्रेडिट कार्ड की जानकारी तक पहुंच है।
जाँच करना
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उपवास
यह उत्पाद केवल शोध उद्देश्यों के लिए है और मनुष्यों या जानवरों में चिकित्सीय या नैदानिक उपयोग के लिए अभिप्रेत नहीं है। उत्पाद और सामग्री येसेन बायोटेक्नोलॉजी के स्वामित्व वाले पेटेंट, ट्रेडमार्क और कॉपीराइट द्वारा संरक्षित हैं। ट्रेडमार्क प्रतीक मूल देश को इंगित करते हैं, जरूरी नहीं कि सभी क्षेत्रों में पंजीकरण हो।
कुछ अनुप्रयोगों के लिए अतिरिक्त तृतीय-पक्ष बौद्धिक संपदा अधिकारों की आवश्यकता हो सकती है।
येसेन नैतिक विज्ञान के प्रति समर्पित हैं, उनका मानना है कि हमारे शोध में सुरक्षा और नैतिक मानकों को सुनिश्चित करते हुए महत्वपूर्ण प्रश्नों का समाधान किया जाना चाहिए।