Description
IL-3, also known as Interleukin-3, is a cytokine that is produced by activated T cells and mast cells. It plays a crucial role in regulating the growth, differentiation, and function of various cell types in the immune system. human IL-3 is an important cytokine that regulates the growth, differentiation, and function of immune cells. Its study contributes to our understanding of immune system regulation and its potential applications in immunotherapy and disease treatment.
Product Properties
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Synonyms |
IL3, MCGF, MGC79398, MGC79399, MULTI-CSF, Interleukin-3 |
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Uniprot No. |
P08700-1 |
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Source |
HEK293 cells-derived human IL-3 protein, Ala20-Phe152, with N-terminal flag tag. |
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Molecular Weight |
The protein has a predicted MW of 16 kDa. And it migrates as 20-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
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Purity |
> 90% as determined by SDS-PAGE. |
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Biological Activity |
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
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Endotoxin |
< 1 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS. |
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Reconstitution |
Centrifuge tubes before opening. Dissolve lyophilized protein with PBS to ensure the concentration is greater than 100 ug/mL.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. |
Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Cautions
1.Please operate with lab coats and disposable gloves, for your safety.
2.This product is for research use only.