Descrição
Specifications
|
Synonyms |
NAG-1; MIC-1;GDF-15; PLAB |
|
Source |
Cynomolgus GDF15 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Arg193-Val308.[Accession | G7PWZ3] |
|
Source |
The protein has a predicted MW of 14.15 kDa. The protein migrates to 15-18 kDa based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE |
|
Activity |
ELISA Data: Immobilized Cynomolgus GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA .Immobilized Cynomolgus GDF15, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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Investigação
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