描述
Interleukin-5 (IL-5) is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils.IL-5 is produced by mast cells, T-cells, and eosinophils, and plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors.Elevated levels of IL-5 can lead to eosinophilia, which may cause asthma and other allergic diseases. Human and mouse IL-5 are cross-species reactive.
Recombinant Human IL-5 is a 26.5 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains. This product, Recombinant Human IL-5, is supplied as a lyophilized powder with high activity, high purity and low endotoxin.
Specification
|
Synonyms |
Interleukin-5; B-cell differentiation factor I, Eosinophil differentiation factor, TRF |
|
Source |
E.coli-derived human IL-5 protein, Ile20-Ser134,with an N-terminal Met. |
|
Accession |
P05113 |
|
Molecular Weight |
Approximately 26.5 kDa. |
|
AA Sequence |
MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 98% by SDS-PAGE and HPLC. |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.5, 3% trehalose, 0.1% Tween-80. |
|
Physical Appearance |
White lyophilized powder |
Storage
-25 ~ -15 ° C storage, valid for 1 year after receipt.
After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolution, store at -85~-65°C, 3 months expiration date.
Compounding Methods
Centrifuge before opening the cap to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier protein (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
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