描述
|
Product Name |
Cat# |
Size |
|
Recombinant Human IL-10 Protein |
90109ES05 |
2 μg |
|
90109ES10 |
10 μg |
|
|
90109ES50 |
50 μg |
|
|
90109ES60 |
100 μg |
|
|
90109ES76 |
500 μg |
Interleukin 10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types, including macrophages, monocytes, T cells, B cells, and keratin-forming cells.IL-10 inhibits the expression of pro-inflammatory cytokines such as IL-1 and TNF-alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune responses.
This recombinant human IL-10 is supplied as a lyophilized powder with high activity, high purity, low endotoxin, and no redundant labeling.
Specification
|
Synonyms |
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, IL10A, TGIF, CSIF；IL10 |
|
Source |
E.coli-derived human IL-10 protein, Ser19-Asn178. |
|
Accession |
P22301 |
|
Molecular Weight |
Approximately 18.6 kDa. |
|
AA Sequence |
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 95%, SDS-PAGE and HPLC detection. |
|
Biological Activity |
The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Physical Appearance |
White lyophilized powder |
Storage
-25 ~ -15 ° C storage, 1 year expiration date after receipt.
After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date.
Compounding Methods
Centrifuge before opening the cap to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier protein (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
付款和安全
您的付款信息已安全處理。我們無法存儲信用卡詳細信息，也無法訪問您的信用卡信息。
詢問
你也可能喜歡
常問問題
本產品僅用於研究目的，不用於人類或動物的治療或診斷用途。產品和內容受以下公司的專利、商標和版權保護：
某些應用程式可能需要額外的第三方智慧財產權。