描述
Specifications
|
Synonyms |
ARP2; ANGPTL2; angiopoietin-like 2; MGC8889 |
|
Source |
Biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Thr260-His493.[Accession | Q9UKU9-1] |
|
Molecular Weight |
The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Tris-Bis PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
SPR Data: Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human ANGPTL2, His Tag with an affinity constant of 65.63 nM as determined in SPR assay . |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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