描述
CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen. Human CD33 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucinlike transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
Specifications
|
Synonyms |
CD34; RP11-328D5.2; CD34 molecule; HPCA1 |
|
Uniprot No. |
P28906.1 |
|
Source |
Recombinant Human CD34 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Ser32-Thr290. |
|
Molecular Weight |
Approximately 28.6 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
ELISA Data: Immobilized Human CD34, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
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