Beschreibung
Specifications
|
Source |
Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-terminal. It contains Met113-Leu261.[Accession | P29965]. |
|
Molecular Weight |
The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on SDS- PAGE result. |
|
Endotoxin |
<1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS- PAGE and HPLC |
|
Activity |
ELISA Data: Immobilized Human CD40 Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 1.29μg/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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FAQ
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