Protein A and Protein G are the gold standard proteins for antibody affinity purification and immunoprecipitation (IP). By covalently coupling both Protein A and Protein G to agarose or magnetic beads, these tools offer a broader binding range compared to beads with only one protein type, enhancing their versatility. Additionally, the Protein A and Protein G used in these products are genetically engineered to retain their high affinity for immunoglobulins (Ig) while eliminating non-primary binding domains, thereby reducing non-specific interactions.
Table 1：Comparison of Protein A/G Agarose Beads and Magnetic Beads
|
|
rProtein A/G Agarose Resin |
Protein A/G MagBeads |
|
Matrix |
Highly cross-linked 4% agarose microspheres |
Silica-based magnetic beads |
|
Particle Size |
45-165 µm |
200 nm |
|
Capacity |
>20 mg hIgG/mL resin |
>0.7 mg hIgG/mL magnetic beads |
|
Advantages |
Easy to use, no auxiliary equipment required |
Simple operation |
|
Large pore size, strong binding force |
Small diameter, good kinetics |
|
|
High yield of target protein |
Smooth surface, minimal bead adsorption, low background, less antibody consumption |
|
|
Disadvantages |
Porous and prone to adsorption (pre-clear required) |
Convenient to use with a magnetic rack |
Product Applications
1. Purification of Antibodies
Isolate high-purity antibodies from serum or ascites in a single step.
2. Immunoprecipitation (IP)
Enrich specific proteins to study its characteristics (such as post-translational modifications).
3. Co-Immunoprecipitation (Co-IP)
Enrich target protein to investigate other proteins that bind to it (i.e., protein-protein interactions, protein complexes, etc.).
Operation Procedure
Test Data
1. Purification of antibodies from rabbit serum using rProtein A/G Agarose Resin.
 |
M: Protein Marker L: Pre-column crude sample elu: Elution |
Figure 1. Electrophoresis result of antibody purification using rProtein A/G Agarose Resin.
2. Antibody Conjugation Experiment with Protein A/G Magbeads
 |
1: NC 2: Yeasen Protein A/G Magbeads 3: Yeasen Protein A/G Magbeads 4: Competitor |
Figure 2. Yeasen Protein A/G Magbeads outperformed Competitor magnetic beads.
3. Use of Protein A/G Magbeads for IP
 |
Target Protein 1: NC 2: Yeasen Protein A/G Magbeads 3: Yeasen Protein A/G Magbeads 4: Competitor |
Figure 3. Yeasen Protein A/G Magbeads outperformed Competitor magnetic beads.
4. Use of Protein A/G Magbeads for Co-IP: Specific antibodies against p65 and cyclin-dependent kinase 9 (CDK9) are used for immunoprecipitation analysis of extracted total cellular protein.
Figure 4. Immunoprecipitants were then subjected to western blot analysis using specific antibodies against CDK9 and p65.
Product Information
|
Product Name |
Product Number |
Specification |
|
Protein A/G Magbeads |
1 mL/5 mL |
|
|
rProtein A/G Agarose Resin |
36403ES08/25/60 |
5 mL/25 mL/100 mL |
Related Products
|
Product Name |
Product Number |
Specification |
|
SuRi rProtein A Agarose Resin |
5 mL/25 mL/100 mL |
|
|
rProtein G Agarose Resin |
36405ES08/25/60 |
5 mL/25 mL/100 mL |