Taq DNA polymerase, as a core enzyme reagent in molecular diagnostics, plays an essential role. However, with the expansion of application fields and the increase in market demand, the limitations of wild-type Taq DNA polymerase have gradually become apparent, such as low amplification efficiency, poor specificity, and poor stability. The Yeasen ZymeEditor™ enzyme modification platform has comprehensively and directionally modified Taq DNA polymerase to meet various application needs, and has obtained a series of Taq DNA polymerase products with a complete range and excellent performance.
Yeasen Hotstart E-Taq DNA Polymerase
Description
Hieff UNICONTM Hotstart E-Taq DNA Polymerase (Cat#10726ES) is a hot start DNA polymerase that employs the company's independently developed dual monoclonal antibodies for double closure. This product not only seals the 5'→3' polymerase activity of Taq DNA polymerase but also seals the 5'→3' exonuclease activity. Heating at the pre-denaturation temperature for 30 seconds can completely inactivate the closure antibodies, releasing the DNA polymerase activity and exonuclease activity. The dual closure feature not only effectively prevents non-specific amplification caused by mispairing or primer dimers but also effectively inhibits the decrease in fluorescence signals caused by probe degradation, providing dual protection to make in vitro diagnostic reagents more stable during transportation or use at room temperature. This Taq enzyme supports the development of a primer-probe pre-mixed system and offers higher sensitivity, specificity, and high GC sequence amplification capability compared to traditional qPCR reagents.
Product Advantages
- High Specificity: Capable of dual encapsulation, it simultaneously inhibits the 5'-3' Taq DNA polymerase activity and the 5'-3' exonuclease activity at room temperature, enhancing the specificity of the reaction.
- No Nuclease Contamination:Free from contamination of exonucleases, endonucleases, and RNases.
- Suitable for High-Sensitivity PCR Experiments:Exhibits a good linear relationship when the template concentration ranges from 5×10^6 copies/T to 5×10^1 copies/T, with the lowest detection limit reaching 5 copies/T.
- Superior Stability:When formulated into a complete premix containing primers and probes, it remains stable after being left at 37°C for 14 days and after 50 freeze-thaw cycles.
- Compatible with Rapid Amplification Protocols: 45 cycles, results within 30 minutes.
Product performance
（1）Good Sensitivity and Linear Range
Figure shows that the qPCR master mix prepared with 10726 exhibits good linearity when the template concentration ranges from 5×10^6 copies/T to 5×10^1 copies/T，R2=0.9997.
（2）Excellent Thermal Accelerated Stability
Figure 3 demonstrates the stability performance of qPCR master mix prepared with 10726ES, using ASFV plasmid DNA as a template under various temperature and time conditions. The results indicate that the qPCR master mix formulated with 10726ES maintains stable performance.
（3）Compatible with Rapid Cycling Programs
Figure shows that the qPCR master mix prepared with 10726ES, using ASFV plasmid DNA as a template, can stably amplify and detect the limit concentration of template DNA（5 copies/T） under both standard（80min） and fast（30min） amplification programs.
Yeasen Biotech, with years of experience in the field of enzyme raw materials, has been providing high-quality and cost-effective molecular diagnostic PCR raw materials to IVD companies, third-party testing institutions, and research organizations. These include single components such as reverse transcriptase, RNase inhibitor, Taq DNA polymerase, uracil DNA glycosylase (UDG/UNG enzyme), Taq enzyme antibody, dNTPs, as well as system-optimized qPCR/RT-qPCR premix solutions, offering superior performance and stable enzyme raw materials for the development of clinical/non-clinical diagnostic kits.
Product recommendation
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产品类型 |
产品名称 |
货号 |
|
PCR enzyme series |
Hieff UNICON® Hotstart E-Taq DNA Polymerase, 5 U/μL |
10726ES |
|
Hifair® V Reverse Transcriptase(200 U/μL) |
11300ES |
|
|
Uracil DNA Glycosylase(UDG/UNG),heat-labile,1 U/μL |
10303ES |
|
|
Murine RNase Inhibitor(40 U/µL) |
10603ES |
|
|
dNTP Mix(25 mM each) |
10125ES |
|
|
Ultra-clean PCR single enzyme series with ultra-low host residue |
Hieff UCF.ME® Hotstart Sensitive Taq DNA Polymerase (5 U/μL) |
14314ES |
|
Hifair UCF.ME® V Reverse Transcriptase (200 U/μL) |
14608ES |
|
|
UCF.ME® Uracil DNA Glycosylase(UDG/UNG), heat-labile, 1 U/μL |
14466ES |
|
|
UCF.ME® Murine RNase Inhibitor(40 U/µL) |
14672ES |
|
|
Lyo-Ready PCR monoenzyme series |
Hieff UNICON® HotStart E-Taq DNA Polymerase, Glycerol-free (5U/μL) |
14316ES |
|
Hifair® V Reverse Transcriptase, Glycerol-free(600 U/μL) |
11301ES |
|
|
Uracil DNA Glycosylase(UDG),Heat-Labile(1 U/μL,Glycerol-Free) |
10707ES |
|
|
Murine RNase Inhibitor(200 U/µL,Glycerol-free) |
10703ES |